ydaF [2018-02-09T22:07:36.000Z]

similar to acetyltransferase

locus: BSU04210

pI: 5.56

mw: 20.88 kDa

protein length: 183 aa

gene length: 549 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 473,174 → 473,725

The protein

Protein family

• N-acetyltransferase domain (according to Swiss-Prot)

Paralogous protein(s)

• [protein|6072A6EF0766F3D1B1616A20CDC36CCDC6CBDA71|YjcK] (36%)

Structure

• [PDB|1NSL] [Pubmed|15468321]

Expression and Regulation

Operons

• genes: [gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]
  description: [Pubmed|22383849]

• genes: [gene|3161519994609DA9360ED6E073E4A4C8C6210EFB|ydaD]-[gene|DDE975DA2E13759B0DB8B6D219760C2E2C6032FB|ydaE]-[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]-[gene|29342783A375260C5DAE920FBF6EC6EFD2D54DDE|ydaG]
  description: [Pubmed|10220166]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528,10220166], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-C088 (ydaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2086 NBRP B. subtilis, Japan]
• BKE04210 (Δ[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCCTCGCCCTCTTTCC, downstream forward: _UP4_TAAAGAGCACACTTTCTTGT
• BKK04210 (Δ[gene|BFDAB855DC76A78C100DC5E5FB81F73069F5F93F|ydaF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCCTCGCCCTCTTTCC, downstream forward: _UP4_TAAAGAGCACACTTTCTTGT

References

• 10220166,18840696,15468321